Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach

Authors : Zeynep DOĞAN, Yasin GENÇ, Şebnem HARPUT, Asuman KARADENİZ PEKGÖZ, İclal SARAÇOĞLU

Pages : 372-377

View : 39 | Download : 12

Publication Date : 2021-12-29

Article Type : Research Paper

Abstract :Background and Aims: Genus Veronica insert ignore into journalissuearticles values(Plantaginaceae); is represented by 79 species in Turkish flora, 26 of which are endemic. Veronica species have a variety of uses including diuretic, anticancer, and rheumatic pains, wounds, and respiratory problems. According to phytochemical studies, Veronica species contain predominantly iridoid glucosides with some phenylethanoid and flavonoid glycosides. Methods: The aqueous extract of Veronica peduncularis M.Bieb. was tested for its cytotoxic activity on human rhabdomyosarcoma insert ignore into journalissuearticles values(RD); and human epidermoid carcinoma insert ignore into journalissuearticles values(HEp-2); cell lines using the MTT method. Chemical profile of the extract was determined by HPLC-DAD, and isolation studies were conducted. Results: The extract was found to show concentration-dependent cytotoxicity against tested cell lines. In addition, a comparison of the iridoid fraction of Veronica peduncularis with previously isolated iridoid glucosides on the HPLC-DAD system, showed the presence of aucubin, amphicoside, veratroyl catalpol, and veronicoside in this fraction. On the other hand, isolation and structure elucidation of plantamajoside and 4`-O-methylisoscutellarein-7-O-2``-O-insert ignore into journalissuearticles values(6```-O-acetyl-ß-D-allopyranosyl);- ß-D-glucopyranoside from the phenolic fractions were performed by serial chromatographic and spectroscopic methods. Conclusion: To the best of our knowledge, this is the first cytotoxic activity and phytochemical study on the titled plant. The presence of iridoid glucosides and 8-hydroxyflavone glycosides is important for the chemotaxonomy of the genus Veronica.
Keywords : 4, HPLC, iridoid glucosides, phenolics, plantamajoside, Veronica

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