Cytotoxicity and genotoxicity evaluations of oleic acid and conjugated linoleic acid

Authors : Sadi KIZILŞAHİN, Çinel KÖKSAL KARAYILDIRIM, Buket BAKAN, Ayşe NALBANTSOY, Ülkü KARABAY
Pages : 14-19
Doi:10.26650/IstanbulJPharm.2022.1023891
View : 48 | Download : 9
Publication Date : 2022-04-28
Article Type : Research Paper
Abstract :Background and Aims: Oleic acid insert ignore into journalissuearticles values(OLA); and conjugated linoleic acid insert ignore into journalissuearticles values(CLA); occur in dairy products and meats and are also widespread at lower levels in many other foodstuffs. It is known that OLA and CLA are very bioactive compounds with sub- stantially anti-carcinogenic effects. The objective of this study was to evaluate the cytotoxic potentials of OLA and CLA which were tested against cancerous and non-cancerous cell lines and to determine their genotoxicity. Methods: The cytotoxic activities of OLA and CLA against to cancer cell lines insert ignore into journalissuearticles values(U-87-MG, A549, MCF-7, CaCo-2, HeLa and PC- 3); and a control cell line insert ignore into journalissuearticles values(HEK293); were assessed by MTT assay. Ames MPFtm mutagenicity assay on 4 strains insert ignore into journalissuearticles values(TA98, TA100, TA 1535 and TA 1537); of Salmonella typhimurium was used for genotoxicity determination. Results: CLA showed cytotoxic activity on PC-3 cells, while OLA was created on A549 and PC-3 cell lines with the IC50 of 20 nM and 15 nM, respectively. No cytotoxic activity was observed on MCF-7, HeLa, U-87-MG, and CaCo-2 cells with the ad- ministered doses of OLA and CLA. It has been proved that OLA and CLA are characterized by a high cytotoxic activity towards cancer cells, as observed in the cell line test. There was no evidence for a mutagenic effect of OLA and CLA in the Ames test, with or without metabolic activation insert ignore into journalissuearticles values(S9); against Salmonella typhimurium strains. Conclusion: These in vitro test results indicate that these fatty acids can be considered a beneficial dietary supplement for enhancing anti-cancer therapy.
Keywords : Fatty acid, Conjugated linoleic acid, Oleic acid, Cytotoxicity, Mutagenicity

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