Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species

Authors : Mehmet AKDENİZ, İsmail YENER, Şafak ÖZHAN KOCAKAYA, Murat YOLCU, Serkan YİĞİTKAN, Fırat AYDIN, Fatma Pınar TÜRKMENOĞLU, Abdulselam ERTAŞ
Pages : 177-185
Doi:10.26650/IstanbulJPharm.2023.1024145
View : 51 | Download : 30
Publication Date : 2023-08-30
Article Type : Research Paper
Abstract :Background and Aims: The importance of Hypericum species that are used traditionally against many diseases is increasing day by day. Methods: In this study, the essential oil contents of Hypericum triquetrifolium, H. empetrifolium subsp. empetrifolium, and H. pruinatum species were determined with GC-MS/FID. This is the first study on the antioxidant, anticholinesterase, antiurease, antityrosinase, antielastase, and anticollagenase activities of these species. Also, in silico and in vitro enzyme inhibitory activities of the major compounds in the essential oil samples of the species have been evaluated. In addition, the cytotoxic effects of the essential oils were determined by the MTT method. Results: According to GC-MS/FID results, the major compounds were determined as caryophyllene oxide insert ignore into journalissuearticles values(16.76%); for H. triquetrifolium, α-pinene insert ignore into journalissuearticles values(21.67%); for H. empetrifolium subsp. empetrifolium, and germacrene D insert ignore into journalissuearticles values(22.47%); for H. pruinatum. Especially, H. pruinatum sample showed a high cytotoxic effect insert ignore into journalissuearticles values(IC50: 34.78±0.22 and 29.06±0.40 µg/mL, respectively); on HT29 and MCF-7 cell lines. It was determined that the same sample showed a promising inhibitory activity on acetyl insert ignore into journalissuearticles values(18.33±2.79, 36.48±2.40, and 56.97±0.94, respectively); and butyryl insert ignore into journalissuearticles values(71.63±2.78, 73.88±1.16, and 56.97±0.97, respectively); cholinesterase enzymes. Conclusion: Results of the in-vitro activity studies indicated that H. pruinatum essential oil could be used in the pharmaceutical industry.
Keywords : Hypericum triquetrifolium, H empetrifolium, H pruinatum, essential oil, cytotoxicity, anti aging, in silico

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