Anti-inflammatory activity of a novel lectin isolated from Pleurotus eryngii var. ferulae mushroom

Authors : Thoiba Mogahid Hussein Seliman, Gülsüm Altıparmak Ülbegi, Nurten Özsoy, Hevidar Alp Kavlo, Pınar Aksoy Sağırlı

Pages : 195-204

Doi:10.26650/IstanbulJPharm.2024.1322171

View : 66 | Download : 93

Publication Date : 2024-08-26

Article Type : Research Paper

Abstract :Background and Aims: Pleurotus species are edible mushrooms with important economic and medicinal value. Many phar macological properties of these species, such as anticancer, immunomodulating, and anti-inflammatory activities, have been attributed to lectin. The aim of this study was to purify a novel lectin from Pleurotus eryngii var. ferulae (PEFL) and investigate its anti-inflammatory activity. Methods:PEFLwaspurifiedby80%ammoniumsulphatefractionation,diethylaminoethyl(DEAE)-Sepharose-4Banionexchange, and Sephadex G-100 gel filtration chromatography. The molar mass of the purified lectin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions. Inhibition of lectin-induced haemagglu tination by several carbohydrates and one glycoprotein (ovalbumin) was also performed using the haemagglutination inhibition activity test. The anti-inflammatory effect of PEFL was tested in a lipopolysaccharide (LPS)-induced inflammation model in mouse macrophage cells (RAW 264.7). The levels of prostaglandin (PG)-E2, tumour necrosis factor-alpha (TNF-
Keywords : Cytokines, Lectin, Lipopolysaccharide, Mushroom, Pleurotus eryngii var ferulae, RAW 264 7 macrophage

ORIGINAL ARTICLE URL