Electron Microscopic and Morphometric Analysis of Wistar Rat Sperm

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Journal of Anatolian Environmental and Animal Sciences
Volume:9 Issue:2
Electron Microscopic and Morphometric Analysis of Wistar Rat Sperm

Electron Microscopic and Morphometric Analysis of Wistar Rat Sperm

Pages : 157-161

View : 88 | Download : 63

Publication Date : 2024-06-30

Article Type : Research Paper

Abstract :The aim of the study is to image and morphologically examine epididymal sperm in Wistar rats with scanning electron microscopy (SEM). The material of the study was epididymal sperm taken from three Wistar Rats. Routine fixation was performed on the obtained epididymal sperm using 2.5% glutaraldehyde solution. Morphometric measurements of sperm were carried out using scanning electron microscopy. The study focused on examining various regions of the sperm, including the head, midpiece, and the remainder of the flagellar segment. Measurements were taken into account in semen that preserved its integrity in different fields. Measurements of these regions were made with the ImageJ program. As a result of the measurements, it was determined that the average head length of rat semen was 7.821 ± 0.844 μm, the average total flagellum length was 168.549 ± 9.19 μm and the average flagellum/head ratio was 21.550 and the flagellum was quite long. It was observed that the measurements of the rat sperm used in the study were consistent with the measurements of other rat sperm. When the studies were examined, it was concluded that rat sperm has an extremely long flagellum compared to its body size. The morphological differences of the sperms revealed will be a guide for experimental studies.
Keywords : Elektron Mikroskop, Sıçan Sperma, Morfoloji

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