Transcriptional expression profiling of feline squamous cell carcinomas

Authors : Mahmut Sözmen, Yonca Betil Kabak, Sinem İnal, Fatma Betül Akça

Pages : 861-868

Doi:10.35229/jaes.1775035

View : 56 | Download : 165

Publication Date : 2025-11-30

Article Type : Research Paper

Abstract :Squamous cell carcinoma (SCC) is the most common malignant cutaneous and oral tumors occurring in cats. Genomic alterations in feline squamous cell carcinomas (FSCC) have not been characterized in cats. The objective of the present study was to determine genes of which expression was significantly altered in naturally occurring FSCC cases by microarray analysis. Two formalin fixed paraffin embedded (FFPE) FSCC cases were analyzed with GeneChipTM Feline Gene 1.0 ST Array. Microarray analysis revealed that FSCC cases harbored 148 differentially expressed genes out of 35,034 gene-level probe sets. Of these changes, 136 genes were up-regulated while the other 12 genes were down-regulated. Microarray analysis results revealed that most of the upregulated genes were oncogenic. Expression levels significantly elevated genes (NCK1, PDE7A, UBAP2L, PPP2R5A, and CDK1) which may have a role in the molecular events leading to the development of FSCC were selected and further examined by real-time PCR in a total of 26 FFPE FSCC cases retrieved from departmental archives. Accordingly, in those 26 FFPE FSCC cases, the fold changes for the NCK1, PDE7A, UBAP2L, PPP2R5A, and CDK1 genes were 6.96, 2.58, 5.24, 55.13, 3.5, respectively. Microarray analysis and real-time PCR results indicate that upregulated genes are involved in the cell cycle and proliferation may also contribute to the development of FSCC.
Keywords : gen ifadesi, kedi, mikroarray, skuamöz hücreli karsinom.

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