Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Authors : Ayşegül ÖZER, Erva RAKICI, Kadriye INAN BEKTAS, Sabriye ÇANAKÇI, Ali Osman BELDUZ

Pages : 30-45

Doi:10.35206/jan.554729

View : 44 | Download : 10

Publication Date : 2020-04-23

Article Type : Research Paper

Abstract :Nine lignin-degrading bacteria were isolated from petroleum-contaminated soil and animal manure samples and characterized by 16S rRNA sequence analysis. Three isolates were identified as Enterobacter cancerogenus , two as Enterobacter ludwigii , one as Citrobacter sedlakii , one as Citrobacter farmeri , one as Klebsiella pneumoniae , and one as Citrobacter murliniae . These  bacteria used ligno sulphate as the sole carbon source but  did not utilize kraft lignin insert ignore into journalissuearticles values(KL); as the sole source of carbon and energy. For this reason, basic nutrients, such as 1.0% glucose insert ignore into journalissuearticles values(w/v); and 0.5% peptone insert ignore into journalissuearticles values(w/v);, were used as additional carbon and nitrogen sources to stimulate bacterial growth for KL decolorization. Under these conditions, the isolates L1, L2, L3, L4, PT21, PT22, PT41, G1, and C1 degraded kraft lignin by 37 %, 14 %, 20%, 43%, 48%, 51%, 28%, 60%, and %99, respectively. The decolorization of Remazol Brilliant Blue R insert ignore into journalissuearticles values(RBBR); by the isolates was analyzed. The isolates were decolorized at 20-90 % of RBBR, respectively. 
Keywords : Lignin, Bacteria, 16S rRNA

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