N-Nitro-L-Arginine Methyl Ester Hydrochloride Induced Endothelial Dysfunction and Atherosclerosis Model in Rats

Authors : Muhammet Kürşat ŞİMŞEK, Mustafa Mahmut BARIŞ, Osman YILMAZ, Zekiye ALTUN, Safiye AKTAŞ, Yasemin CAKİR, Sibel BÜYÜKÇOBAN, Mustafa SEÇİL
Pages : 114-121
Doi:10.30621/jbachs.1226509
View : 59 | Download : 39
Publication Date : 2023-09-30
Article Type : Research Paper
Abstract :Purpose: Atherosclerosis insert ignore into journalissuearticles values(AS); related diseases are the most common causes of mortality worldwide. N-Nitro-L-Arginine Methyl Ester Hydrochloride insert ignore into journalissuearticles values(L-NAME);-induced endothelial dysfunction insert ignore into journalissuearticles values(ED); and AS models require invasive methods for diagnosis. We aimed to establish noninvasive ultrasonography insert ignore into journalissuearticles values(USG); model for evaluating ED and AS in rats. Matherial and Methods: 23 Wistar Albino rats were divided into four groups. Right CCA insert ignore into journalissuearticles values(rCCA);, left CCA insert ignore into journalissuearticles values(lCCA);, abdominal aorta insert ignore into journalissuearticles values(AA);, and right iliac artery insert ignore into journalissuearticles values(rIA); IMT values of all rats were measured at the beginning of the experiment and before sacrification by USG. Right kidney RI values were calculated at similar times also. Histopathological and immunohistochemical analyzes were performed. In the sham group, rats received intraperitoneally insert ignore into journalissuearticles values(IP); sodium chloride. In the L-NAME groups, IP L-NAME was administered. Results: In the early effect group, significant increase was found in IMT measurements compared to the sham group. In the late effect group, significant increase was found in IMT measurements compared to the sham group. In addition, rRI increased significantly in the group at the end of the experiment. Conclusion: In small animal experiments which ED and AS were studied, a whole-body diagnostic noninvasive model was created for the first time with ultrasonography.
Keywords : Ultrasound, Intima media tickness, L NAME, renal resistive index

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