Comparative Evaluation of the Effects of Sevoflurane and/or Dexmedetomidine on Behavior, Neuro-inflammation and Apoptosis in Pups Rat

Authors : Semin TURHAN, Arda İNAN, Berrin IŞIK

Pages : 657-664

Doi:10.16899/jcm.1297757

View : 63 | Download : 88

Publication Date : 2023-07-31

Article Type : Research Paper

Abstract :Aim: Developing brain is vulnerable to side effects of anesthetics. Neurotoxic and cognitive alterations have been documented in several species, and there is concern that small children could be affected adversely if they are exposed for long periods or recurrently to inhalation anesthesia In this experiment we aim to evaluate behavioral and neurotoxic effects of sevoflurane insert ignore into journalissuearticles values(SEVO); and/or dexmedetomidine insert ignore into journalissuearticles values(DEX); exposure in pup rats. Materials and method: Postnatal 21 days old 36 rat were randomly divided into 6 groups insert ignore into journalissuearticles values(Group I insert ignore into journalissuearticles values(control);; Group II:2.5% SEVO for 4 hours; Group III:2.5% SEVO for 4 hours+intraperitoneal insert ignore into journalissuearticles values(i.p.); 0.5 µg.kg-1 DEX; Group IV:2.5% SEVO for 4 hours+i.p. 5 µg.kg-1 DEX; Group V: i.p. 0.5 µg.kg-1 DEX; Group VI: i.p. 5 µg.kg-1 DEX was given);. Behavior of the rat were examined with the modified Radial Arm Maze test. Histopathological evaluation of the pups’ rat brain for neuroinflammation and apoptosis was performed. Statistical evaluation was carried out using the SPSS 20.0, P value <0.05 was considered statistically significant. Results: Single 2.5% SEVO exposure for 4 hours during early life period in rats is although not show neuroinflammation signs the brain tissue histologically but impaired learning and memory in behavior test insert ignore into journalissuearticles values(P<0.05);. In CA3 stage of the brain tissue apoptosis percentage was diminished in SEVO+DEX groups for comparison with control and single SEVO groups insert ignore into journalissuearticles values(P<0.05);. Conclusions: Adding DEX to SEVO caused less impairment in memory and learning function. But single 5 µg.kg-1 DEX negatively affected learning and memory function but not locomotor activity and anxiety.
Keywords : sevofluran, deksmedetomidin, yavru fareler, nörotoksik, kognitif değişiklikler

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