Correlation between anti-dsDNA, complement components C3, C4 and Systemeic Lupus Erythematosus disease activity index

Authors : Therese Mary DHASON

Pages : 54-61

Doi:10.5455/jicm.26-20170707

View : 62 | Download : 29

Publication Date : 2017-09-30

Article Type : Research Paper

Abstract :Background: To find out the correlation between anti-ds DNA, complement components C3, C34 and disease activity in SLE. Material and Methods: This cross-sectional study was undertaken over a period of 6 months from January 2016 to June 2016 at a Tertiary Care Centre in South India.  Blood samples were collected from 100 patients with active lupus, 100 patients with inactive disease and 100 healthy controls. Serum samples from all the 300 samples were tested for anti- dsDNA by ELISA and C3, C4 by Radial Immunodiffusion. Results: High titre anti-dsDNA was detected in 48 out of the 100 patients with active disease. Mean SLE disease activity index insert ignore into journalissuearticles values(SLEDAI); of patients with high titre anti-dsDNA was 23.08337 ± 3.80276 insert ignore into journalissuearticles values(P<0.001); Mean SLEDAI of patients with very low C3 and C4 was 21.4314 ± 4.88776 insert ignore into journalissuearticles values(P<0.001); and  21.1250 ± 4.82882 insert ignore into journalissuearticles values(P<0.001);.  Conclusion: Anti-dsDNA, C3, C4 levels can be adopted as markers of disease activity in SLE. Anti-dsDNA by ELISA is a simple and cost effective method. Radial immunodiffusion can be done for C3 and C4 assay where nephelometer is not available as the procedure is simple.
Keywords : Systemic Lupus Erythematosus, anti ds DNA antibody, ELISA, C3, C4, radial immuno diffusion

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