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  • Journal of Experimental and Clinical Medicine
  • Volume:12 Issue:3
  • Tavşanda N.Fibularis ( Peroneus ) Commus’i Meydana Getiren Motor Nöronların Medulla Spinalisteki Lok...

Tavşanda N.Fibularis ( Peroneus ) Commus’i Meydana Getiren Motor Nöronların Medulla Spinalisteki Lokalizasyonu

Authors : S BİLGİÇ, B ŞAHİN
Pages : 0-0
Doi:10.5835/jecm.v12i3.626
View : 85 | Download : 16
Publication Date : 2009-12-23
Article Type : Other Papers
Abstract :Motoneurones of the peripheral nerves are located in the anterior horn of the spinal cord as longitudinal columns. In this study location of the motoneurones of the common fibular insert ignore into journalissuearticles values(peroneal); nerve was investigated with the Horseradish Peroxidase insert ignore into journalissuearticles values(HRP); method, in 3 male and 3 female rabbits. The animals were anaesthetized with an intramuscular in¬jection of ketamine+xylazine. The common peroneal nerve was exposed surgically and the cut proximal end were soaked into a small container which is filled the 30% solution of insert ignore into journalissuearticles values(HRP); for 1-2 hours. After 48-72 hours survival period the animals were perfussed intra -cardially. Serum physiologic and 2% gluteraldehyde + 2% paraformaldehyde in 0.1M phosphate buffered insert ignore into journalissuearticles values(pH 7.3); were used as a perfusion solution. The spinal cords were cut in a freezing microtome at a thickness of 50 |i in coronal sections. The sries were reacted according to Mesulam`s procedure. The motoneurones were located in the rostral portion of anterior horn between L6-S1 segments of the spinal cord. Motoneurones diameters were 3.47 \x m and it was 238 in number. Periferik spinal sinirlerdeki somatomotor liflerin hücre gövdeleri medulla spinalis insert ignore into journalissuearticles values(MS); gri cevherinin ön boynuzunda bulunur ve uzun sütunlar oluştururlar. Bu çalışmamızda n. fi¬bularis insert ignore into journalissuearticles values(peroneus); communis`i meydana getiren motor nöronların MS`deki lokalizasyonlarını Horseradish Peroxidase insert ignore into journalissuearticles values(HRP); metodu ile inceledik. Hayvanlar xylazine + ketamin enjeksiyonu ile anestezi edildikten sonra n. fibularis communis kesildi ve %30`luk HRP solüsyonu sinire 1-2 saat süre ile emdirildi. Yara usulüne uygun kapatıldı. Hayvanlar 48-72 saat yaşatıldıktan sonra sakrifiye edildiler. Sol ventrikül yoluyla yapılan perfüzyonda serum fizyolojikten sonra pH`sı 7.3 olan fosfat tamponunda %2`lik glutaraldehit, %2`lik paraformaldehit ihtiva eden fiksatif kullanıldı. MS`ten dondurma mikrotomunda 40 \ı kalınlığında kesitler alındı. Histokimyasal metodlar Mesulam`ın tekniğine göre yapıldı. N. fibunaris communis`i meydana getiren motor nöronlar MS`in ön boynuzunun lateral bölgelerinde olmak üzere L6 - Sİ segmentlerinde lokalize olmuşlardı. Motor nöronların çaplan ortalama 34.7 insert ignore into journalissuearticles values(i, sayıları ise ortalama 238 olarak bulundu.
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