The effect of zinc supplementation on cigarette smoke-induced nephrotoxicity in rats

Authors : Arif DURAN, Zeynep OCAK, Tarık OCAK, Hakan TERZİ, Alim ERDEM, Ertuğrul KOCAMAN

Pages : 353-356

Doi:10.5835/jecm.omu.30.04.015

View : 40 | Download : 11

Publication Date : 2014-02-05

Article Type : Research Paper

Abstract :The aim of our study was to investigate the effect of zinc supplemented diet on the cigarette smoke-induced changes on toll-like receptor insert ignore into journalissuearticles values(TLR);, nuclear factor of kappa light polypeptide gene enhancer in B-cells insert ignore into journalissuearticles values(NFKB); and cytokine gene expression in renal tissue of rats. A total of 24 Wistar albino rats were allocated into four groups as group A: Non-smoke controls insert ignore into journalissuearticles values(n:6);, group B: Non-smoke+zinc insert ignore into journalissuearticles values(n:6);, group C: Smoke insert ignore into journalissuearticles values(n:6);, and group D; smoke+zinc insert ignore into journalissuearticles values(n:6);. RNA isolation was performed from the renal tissues of the rats which were sacrificed at the end of the 12th week. The expression levels of TLR2, TLR4, NFKB1, NFKB2 and interleukin 1 alpha insert ignore into journalissuearticles values(IL1A);, interleukin 10 insert ignore into journalissuearticles values(IL10); genes were evaluated using the Reverse Transcription Polymerase Chain Reaction insert ignore into journalissuearticles values(RT-PCR);. In addition, these tissues were examined histopathologically and the direct protective effect of zinc on these tissues was investigated. No significant difference was found between the groups in terms of TLR2 and TLR4, NFKB1 and NFKB2, IL1 and IL10 gene expression levels. As a result of the histopathological assessment, a statistically significant difference was detected between Group A, C and D in terms of hemorrhage, congestion, inflammation, glomerular atrophy and tubular injury insert ignore into journalissuearticles values(p<0.05);. The zinc supplementation may have a protective effect from cigarette smoke-induced nephrotoxicity in rats.
Keywords : Interleukin, Nuclear factor of kappa light polypeptide, Gene enhancer in B cells, Renal injury, Smoke, Toll like receptor, Zinc

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