Assessment of human thyroid function using radioimmunoassay and enzyme-linkedimmuno- sorbent-assay

Authors : Mohamed MOHAMED GARELNAB, Reham TAHA, Mohammed ALİOMER, Mohamed FARAHNA, Yahia M BUSHARA

Pages : 317-321

Doi:10.5835/jecm.omu.30.04.007

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Publication Date : 2014-02-05

Article Type : Other Papers

Abstract :Thyroid function has been evaluated using Radioimmunoassay insert ignore into journalissuearticles values(RIA); and enzyme-linked-immuno-sorbent-assay insert ignore into journalissuearticles values(ELISA); techniques as a comparative study to determine the accuracy, sensitivity and specificity in addition to correlation with body mass index insert ignore into journalissuearticles values(BMI);. For triiodothyronine insert ignore into journalissuearticles values(T3); measured by RIA, relatively ELISA showed sensitivity, specificity and accuracy of 93.8%, 100% and 99.1% respectively and for thyroxin insert ignore into journalissuearticles values(T4);, ELISA showed sensitivity, specificity and accuracy of 79.3%, 89.4% and 86.8% respectively. And for thyroid-stimulating hormone insert ignore into journalissuearticles values(TSH); ELISA showed a sensitivity, specificity and accuracy of 72.4%, 90.6% and 86% respectively and relative to RIA technique which is considered as standard goal. And for compensating the ELISA results to be same as RIA the following coefficient 0.72, 6.8 and 0.23 for T3, T4 and TSH respectively could be used. Also there is inverse linear relationship between the BMI and T3 and T4 using ELISA whereas the amount of T3 and T4 decreases by 0.006 nmol/kg/m2 and 0.051nmol/kg/m2 respectively. But TSH shows a direct linear relationship with BMI where TSH slightly increased by 0.007nmol/kg/m2. J. Exp. Clin. Med., 2013; 30:317-321
Keywords : Radioimmunoassay, enzyme linked immunosorbent assay, thyroid

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