Protective effects of tropisetron on diabetes-induced expression of genes involved in regulation of contractility, cardiomyopathy and apoptosis in the left ventricle of rats

Authors : Roya NADERİ, Alireza SHİRPOOR, Mahrokh SAMADİ, Farideh NEZAMİMAJD, Yousef RASMİ, Morteza BAGHERİ

Pages : 376-382

View : 38 | Download : 11

Publication Date : 2022-03-18

Article Type : Research Paper

Abstract :Tropisetron, an antagonist of 5-HT3 receptor insert ignore into journalissuearticles values(5-HT3R);, has exhibited a number of beneficial effects on the treatment of several diseases; however, its effect on diabetic cardiomyopathy, which is an important causal factor in morbidity and mortality among diabetic patients, remains to be fully elucidated. Therefore, this study was designed to investigate the effect of tropisetron on diabetes-induced cardiomyopathy and the molecular mediators possibly involved. Twenty-four male wistar rats were assigned into three groups, control, diabetic, and tropisetron–treated diabetic groups. After 14 days of treatment, the results revealed a significant increase in calcium/calmodulin-dependent protein kinaseIIδ insert ignore into journalissuearticles values(CaMKIIδ); total and isoforms δ2 and δ3 of CaMKIIδ, as well as myosin heavy chain insert ignore into journalissuearticles values(MHC);-β. Furthermore, it decreased MHC-α gene expression among the diabetic group compared to the control group. Western blot analysis showed a significant increase in Bax and cleaved caspase 3 protein levels and a significant decrease in Bcl-2 contents in heart tissue of diabetic rats in comparison to the control rats. Moreover, the levels of Tumor necrosis factor alpha insert ignore into journalissuearticles values(TNF-α); , ICAM-1, and CaMKII in heart tissue of diabetic rats were significantly higher than those in the control rats. Significant amelioration of alteration in the genes expression and protein changes along with restoration of the elevated levels of TNF-α, ICAM-1, and CaMKII were found in the tropisetron–treated diabetic group compared to the diabetic group. Collectively, our study provided strong evidence that 5-HT3 antagonist tropisetron was capable of attenuating the development of experimental diabetic cardiomyopathy associated with the reduction of intramyocardial MHCs and CaMKIIδ gene expression, inflammation and cardiac hypertrophy.
Keywords : Apoptosis, Diabetes, Tropisetron

ORIGINAL ARTICLE URL