Protective effects of glutathione administration on ischemia-reperfusion injury in rat ovaries

Authors : Ali GÜRSOY, Belkız ONGEN, Ayse Gokcen SADECE, Kemal ATASAYAN, Ezgi Doğan TEKBAŞ, Mustafa Erinc SİTAR

Pages : 1-6

View : 271 | Download : 324

Publication Date : 2023-03-18

Article Type : Research Paper

Abstract :We aimed to examine the biochemical and histopathological potential beneficial effects of glutathione administration on the ovarian ischemia/reperfusion injury insert ignore into journalissuearticles values(IRI); model. Thirty Wistar Albino female rats were used in this experimental study and were divided into five groups. Group 1 insert ignore into journalissuearticles values(sham); underwent observational laparotomy. Group 2 insert ignore into journalissuearticles values(torsion); had their left ovaries torsioned. Group 3 insert ignore into journalissuearticles values(torsion + detorsion); was detorsioned after torsion. Groups 4 and 5 received the same procedure as group 3. 0,2 ml glutathione was applied to the left ovaries of group 4 insert ignore into journalissuearticles values(torsion + detorsion + intraovarian glutathione injection); after detorsion. Group 5 insert ignore into journalissuearticles values(torsion + detorsion + intraperitoneal glutathione injection); was administered 1 ml glutathione intraperitoneally five times. Fifteenth-day blood samples were taken to examine total antioxidant status, total oxidant status, oxidative stress index, anti-mullerian hormone insert ignore into journalissuearticles values(AMH);, and malondialdehyde insert ignore into journalissuearticles values(MDA); values. Besides, the left ovaries were resected for histopathological examination. Total antioxidant status was significantly higher in the intraperitoneal injection group insert ignore into journalissuearticles values(p 0.05);. MDA value did not differ significantly between the sham, intraovarian, and intraperitoneal injection groups insert ignore into journalissuearticles values(p>0.05);. In histopathological examination, no significant benefit of glutathione application on follicle numbers was shown. The main limitations of our study were the relatively small size of our series, the absence of serial blood measurement, the absence of a group in which intraovarian and intraperitoneal injections were administered together, and the absence of a sham + drug group. Glutathione administration reduces the detrimental effects of ovarian IRI.
Keywords : Anti Mullerian Hormone, Glutathione, Ischemia Reperfusion Injury, Ovarian Torsion

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