Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method

Authors : Özhan Karataş, Gökhan Akçakavak, Murat Eren, Zeynep Çelik, Ayşenur Tural, Osman Dağar, Osman Doğan, Bahadır Kılınç, Mehmet Tuzcu
Pages : 9-15
Doi:10.53518/mjavl.1572721
View : 67 | Download : 55
Publication Date : 2025-06-26
Article Type : Research Paper
Abstract :Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2\\\'-deoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE) expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10% formaldehyde solution, they underwent routine tissue processing to obtain paraffin blocks. Sections taken from the paraffin blocks were then subjected to Hematoxylin-Eosin (H-E) and immunohistochemical staining. Microscopically, control group liver samples showed normal histology. In livers with hepatic lipidosis, sharp-edged vacuoles of various sizes were detected in hepatocytes, and cell nuclei were pushed to the periphery. Additionally, focal hemorrhage and congestion, inflammatory cell infiltration in the portal area, bile duct proliferation, and connective tissue cells were observed. Microscopically, hepatic lipidosis cases were divided into two groups as moderate and severe based on the distribution of vacuoles in the section. In the immunohistochemical examination, 8-OHdG and 4-HNE expressions significantly increased in hepatic lipidosis cases compared to the control group (p<0.001). More intense immunoreactivity was detected especially in cases where disease severity increased (p<0.001). These results indicate that 8-OHdG and 4-HNE proteins play an important role in the pathogenesis of hepatic lipidosis and may be effective in increasing the severity of the disease.
Keywords : 8-hydroxy-2, 4-Hydroxynonenal, hepatic lipidosis, sheep

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