Molecular analysis of human adenoviral keratoconjunctivitis cases: Results of a 2-year survey

Authors : Ayfer GUNER, Rabia CAN SARINOGLU, Fahri Onur AYDIN, Semra AKKAYA TURHAN, Mert Ahmet KUSKUCU, Ayse Ebru TOKER, Aysegul KARAHASAN YAGCI

Pages : 1-6

Doi:10.5472/marumj.1244369

View : 43 | Download : 8

Publication Date : 2023-01-31

Article Type : Research Paper

Abstract :Objective: This study aimed to determine the adenovirus genotypes and their epidemiological features between January 2018 and November 2019, in Istanbul, Turkey. Material and Methods: Conjunctival swab samples were obtained from patients who were clinically diagnosed with keratoconjunctivitis. Samples were screened with an Adeno Detector kit insert ignore into journalissuearticles values(Rapid Pathogen Screening, RPS Inc., South Williamsport, PA);. Nucleic acid extraction and amplification were performed with the ADENOVIRUS ELITe MGB® kit in the ELITe In Genius instrument insert ignore into journalissuearticles values(Elitech Group, Torino, Italy);. For subtyping of the strains, sequencing primers targeted the ‘Hypervariable Region 7’ insert ignore into journalissuearticles values(HVR-7); of the hexon gene were used. DNA sequence analysis insert ignore into journalissuearticles values(n:72); was performed with ABI PRISM® 3100 Genetic Analyzer insert ignore into journalissuearticles values(Applied Biosystems, USA);, and subtyping was done by BLAST analysis. Results: The median viral load in the samples insert ignore into journalissuearticles values(n: 77); was 7 log10 copies/mL insert ignore into journalissuearticles values(IQR: 4.5-7.4 log10 copies/mL);. The clinical finding score was found to be significantly higher in the high viral load group insert ignore into journalissuearticles values(Adenovirus DNA≥6 Log 10 copies/mL); than in the low viral load group insert ignore into journalissuearticles values(Adenovirus DNA<6 Log 10 copies/mL); insert ignore into journalissuearticles values(p = 0.031);. Conclusion: Our study analyzing hAdV strains collected in 2018 and 2019 revealed that genotype 8 is the dominant type insert ignore into journalissuearticles values(94.0%); in our region. Molecular methods are very useful for future epidemiological studies and the selection of a vaccine strain.
Keywords : Adenovirus, Genotyping, Viral conjunctivitis, Epidemiological analyzes, Epidemic keratoconjunctivitis, Adenovirus, Genotyping, Viral conjunctivitis, Epidemiological analyzes, Epidemic keratoconjunctivitis

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