GABAergic effects of some foods extracts via inhibition of GABA transaminase (GABA-T)

Authors : Sümeyye ŞAHİN, Sahra HAAS

Pages : 423-428

Doi:10.19127/mbsjohs.976658

View : 44 | Download : 8

Publication Date : 2021-12-31

Article Type : Research Paper

Abstract :Objective: GABAergic system is a target for various groups of medications including sedatives, anxiolytics, muscle relaxants, antidepressants and antiepileptics. Several foods or food ingredients are able to affect the GABAergic system by the inhibition of the γ-aminobutyric acid insert ignore into journalissuearticles values(GABA); degrading enzymes including the GABA-transaminase and succinate semialdehyde dehydrogenase. The purpose of this study to investigate the inhibitory effects of tea insert ignore into journalissuearticles values( Camellia sinensis );, coffee insert ignore into journalissuearticles values( Coffea arabica L. );, peppermint insert ignore into journalissuearticles values( Mentha piperita L. );, thyme insert ignore into journalissuearticles values( Thymus vulgaris L. );, and cinnamon insert ignore into journalissuearticles values( Cinnamomum zeylanicum ); on GABA degrading enzymes. Methods: The inhibition of the GABA-T by aqueous extracts of tea insert ignore into journalissuearticles values( Camellia sinensis );, coffee insert ignore into journalissuearticles values( Coffea arabica L. );, peppermint insert ignore into journalissuearticles values( Mentha piperita L. );, cinnamon insert ignore into journalissuearticles values( Cinnamomum zeylanicum );, and thyme insert ignore into journalissuearticles values( Thymus vulgaris L. ); was investigated using a fluorometric microplate enzyme assay. Dose-dependent inhibition of the GABA-degrading enzymes was attained by all the food extracts tested. For determination of the IC50 values of the extracts insert ignore into journalissuearticles values(± 95 % CI);, a linear regression was performed using Origin® insert ignore into journalissuearticles values(Origin® 2015G von Origin Lab Corporation, Northampton, MA 01060 USA);. Results: The aqueous extract of black tea presented the strongest inhibitory activity with an IC50-value insert ignore into journalissuearticles values(half maximal inhibitory concentration); of 13.0 insert ignore into journalissuearticles values(11.0-15.3); µg/mL. The tested food extracts were successful in inhibiting the GABA-degrading enzymes even at low concentrations. Conclusion: In conclusion, the selected food extracts could serve as natural inhibitors for GABA-degrading enzymes thus, they could increase the GABA concentration in the brain.   
Keywords : γ Amino butyric acid, GABA transaminase, enzyme inhibition, black tea, thyme

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