BIOELECTRICITY GENERATION BY SACCHAROMYCES CEREVISIAE USING METHYLENE BLUE MEDIATOR IN VARIOUS MICROBIAL FUEL CELL DESIGNS

Authors : Gizem Hazan Akçay

Pages : 49-55

Doi:10.22531/muglajsci.1737770

View : 41 | Download : 161

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :The discovery of electroactive microorganisms and their electron transfer mechanisms has opened new possibilities for bioelectrochemical wastewater treatment. Microbial fuel cells (MFCs) generate electricity by converting organic substrates through microbial metabolism. In this study, Saccharomyces cerevisiae was utilized as a biocatalyst to assess the performance of various MFC designs using domestic wastewater as the anode substrate. The effects of reactor configuration and the addition of methylene blue as a redox mediator on voltage generation, power density, and chemical oxygen demand (COD) removal were investigated. Two batch-mode reactors were tested: a nested cylindrical dual-chamber MFC (NDMFC) and a cylindrical H-type MFC (CHMFC). The maximum power densities achieved were 3.22 mW/m² for the NDMFC and 3.35 mW/m² for the CHMFC, while COD removal efficiencies were 90.33% and 91.44%, respectively. As all operational conditions were maintained constant, the observed performance variations were attributed to differences in reactor design. Overall, this study aimed to emphasize the importance of mediators that facilitate electron transfer in microbial fuel cells. The effect of methylene blue on power generation was effectively investigated using different reactor configurations.
Keywords : Saccharomyces cerevisiae, Medyatör, Güç yoğunluğu, Mikrobiyal yakıt hücresi, Biyoteknoloji

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