The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)

Authors : Fulya ÖZDİL, Mehmet Ali YILDIZ

Pages : 46-51

View : 38 | Download : 18

Publication Date : 2008-08-25

Article Type : Research Paper

Abstract :In this study, the identification of Turkish honey bee populations was carried out using cytochrome C oxidase I and II intergenic region of mitochondrial genome. A total of 244 worker bees from 20 different locations in Turkey were used. Restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); and DNA sequence data were utilized to identify the honeybee populations. DraI digestion of the COI-COII intergenic region generated four fragments. In this study, DraI digestion of the COI-COII intergenic region gave rise to a total of five DraI haplotypes which have been accurately identified from DNA sequence results. Three of the DraI haplotypes insert ignore into journalissuearticles values(47/41/64/420 bp-C1; 47/40/64/420 bp-C2 and 47/39/64/420 bp); have been reported in other papers but two of them insert ignore into journalissuearticles values(47/41/64/419 bp and 47/39/64/418 bp); were first seen in this study. DraI digestion of the COI-COII intergenic region generated four fragments in Turkish honeybees which previously have been found to be characteristic of the C lineage an these results confirmed that Turkish honey bee populations belong to East European and Mediterranean insert ignore into journalissuearticles values(C); lineage.
Keywords : Apis mellifera L, mtDNA, COI COII intergenik bölge, PCR RFLP, DNA dizi analizi

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