Beneficial effects of linagliptin in cell culture model of Parkinson’s disease

Authors : Ufuk OKKAY, Irmak FERAH OKKAY

Pages : 242-246

Doi:10.18621/eurj.1010322

View : 32 | Download : 9

Publication Date : 2022-03-04

Article Type : Research Paper

Abstract :Objectives: We aimed to investigate the neuroprotective effects of linagliptin in an in vitro 6-hydroxydopamine insert ignore into journalissuearticles values(6-OHDA); Parkinson’s disease model. Methods: 6-OHDA insert ignore into journalissuearticles values(200 µM); were administered to the SH-SY5Y cells for 24 h to induce Parkinson’s disease model in vitro. Cells were treated with linagliptin insert ignore into journalissuearticles values(1, 10, 50 and 100 nM); 30 minutes before 6-OHDA administration. Cell viability was examined by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide insert ignore into journalissuearticles values(MTT); method and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); analysis. Superoxide dismutase insert ignore into journalissuearticles values(SOD);, catalase insert ignore into journalissuearticles values(CAT);, malondialdehyde insert ignore into journalissuearticles values(MDA); and reactive oxygen species insert ignore into journalissuearticles values(ROS); analyses were conducted to assess oxidative stress. Apoptosis was evaluated with Caspase-3 mRNA expression levels. Results: It was observed that 6-OHDA elevated LDH levels and cell death. Oxidative stress was exaggerated with increased ROS and MDA levels and substantially apoptosis was proven with increased Caspase-3 levels in SH-SY5Y cells. Pretreatment with linagliptin alleviated oxidative stress and apoptosis. Conclusions: Given its neuroprotective role as well as its effects on oxidative stress and apoptosis, linagliptin may be a drug candidate in Parkinson`s disease.
Keywords : 6 OHDA, DPP 4 inhibitor, linagliptin, Parkinsons disease, SH SY5Y cells

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