Investigation of antioxidant and antimicrobial activities of walnut (Juglans regia L.) kernel septum

Authors : Elif Azize ÖZŞAHİN DELİBAŞ, Esin KIRAY

Pages : 87-96

Doi:10.18621/eurj.1056629

View : 36 | Download : 9

Publication Date : 2023-01-04

Article Type : Research Paper

Abstract :Objectives: Walnut insert ignore into journalissuearticles values( Juglans regia L.); kernel septum insert ignore into journalissuearticles values(or septa); insert ignore into journalissuearticles values(WKS);, a traditional nutraceutical material in China, has not been explored in detail. In this study, antimicrobial activity, total phenolic content insert ignore into journalissuearticles values(TPC); and antioxidant-oxidant status of WKS was investigated in case it may be clinically important in the management of various complications. Methods: The WKS was extracted with ethanol in a Soxhlet device. TPC of WKS was analysed by using Folin-Ciocalteu’s method. Antioxidant activity was obtained by using Rel Assay Diagnostics kits. The antimicrobial activity of WKS was evaluated against two Gram-positive insert ignore into journalissuearticles values( Staphylococcus aureus, Bacillus subtilis );, one Gram-negative bacteria insert ignore into journalissuearticles values( Escherichia coli ); and one fungus insert ignore into journalissuearticles values( Candida albicans ); strains using the agar diffusion method. Results: The TPC of WKS was found to be 119.42 ± 2.39 mg GAE/gDW. It was determined that total antioxidant status insert ignore into journalissuearticles values(TAS);, total oxidant status insert ignore into journalissuearticles values(TOS); and oxidative stress index insert ignore into journalissuearticles values(OSI); values were 7.542 ± 0.389 mmol/L, 3.718 ± 0.287 µmol/L and 0.049 ± 0.001, respectively. WKS selectively inhibited the growth of Gram-positive bacteria and fungus, while S. aureus was the most susceptible one with 16 mm of inhibition zone. Gram-negative bacteria was resistant to the extract. Conclusions: As far as we know, this paper is the first work that demonstrates the antioxidant-oxidant status of WKS by using the method described above, and moreover there are no scientific reports which have examined WKS in such a multidisciplinary experimental design. This study strongly supports the reported traditional use of WKS. Results indicated that WKS can be used as a pharmacological natural agent due to its high antioxidant and antimicrobial activities.
Keywords : Juglans regia L, walnut kernel septum, antioxidant, total phenolic content, antimicrobial activity

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