Identification and Molecular Characterization of Genes Related to Terpene Biosynthesis From Lavandula × intermedia

Authors : Damla Önder, Sercan Önder, Yaşar Karakurt

Pages : 103-112

Doi:10.5152/AUAF.2023.23150

View : 73 | Download : 274

Publication Date : 2023-10-19

Article Type : Research Paper

Abstract :Lavandin (Lavandula × intermedia Emeric ex Loisel.) is an essential oil-bearing plant with high economic value for the cosmetics and pharmaceutical industries due to its fragrance components. These essential oils primarily contain monoterpenes, including geraniol, linalool, and linalyl acetate. The essential oil content of Lavandula × intermedia is five times higher than that of Lavandula angustifolia oil. However, due to its relatively high content of linalool and linalyl acetate, the highest quality and sought-after lavender oil is produced from Lavandula angustifolia. In this study, the genes encoding geranyl diphosphate synthase, linalool synthase, and linalyl acetyl transferase were isolated from glandular trichomes of lavandin flowers. The results showed that Lavandula × intermediageranyl diphosphate synthase, Lavandula × intermedia linalool synthase, and Lavandula × intermedia linalyl acetyl transferase demonstrated expressions from the budding to the full blooming stage of flower development, and the expression levels of Lavandula × intermedia linalool synthase were lower in comparison with the expressions of Lavandula × intermedia geranyl diphosphate synthase and Lavandula × intermedia linalyl acetyl transferase. Phylogenetic analyses showed that Lavandula × intermedia linalool synthase and Lavandula angustifolia linalool synthase were at similar levels, while Lavandula × intermedia geranyl diphosphate synthase, Lavandula × intermedia linalyl acetyl transferase, and other lavender species geranyl diphosphate and linalyl acetyl transferase were different. The identification of these terpene synthases from lavandin forms the basis for improving the quality of essential oil composition in lavandin through metabolic engineering.
Keywords : Uçucu yağ, gen klonlama ve ifadesi, geranil difosfat sentaz, lavandin, linalool sentaz, linalil asetil transferaz

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