Mutation dose determination of cherry tomato (Solanum lycopersicum L. cerasiforme) under in vitro and ex vitro conditionsions

Authors : Irmak Çakın, Kadriye Yaprak Kantoğlu, Erhan Aksu

Pages : 21-30

View : 77 | Download : 145

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :In this study, biological responses resulting from gamma irradiation of cherry tomato (Solanum lycopersicum L. var. cerasiforme) seeds under different germination conditions (in vitro and ex vitro) were evaluated, and the effective mutation dose (EMD₅₀) was determined. In the in vitro experiment, seeds were irradiated with gamma rays at doses ranging from 0 to 80 Gy and germinated on Murashige and Skoog (MS) medium without any plant growth regulators. In the ex vitro conditions, seeds were exposed to doses between 0 and 600 Gy and sown in pots filled with a soil mixture. Seed germination was monitored during the first 10 days post-irradiation, and parameters such as shoot length, fresh weight, and dry weight of seedlings were measured at the end of a 30-day incubation period. Regression analyses revealed an EMD₅₀ value of 39.08 Gy under in vitro conditions and 608.6 Gy under ex vitro conditions. The findings demonstrate that the effective irradiation dose varies depending on the irradiation conditions (in vitro vs. ex vitro). These results emphasize the importance of considering the culture conditions when selecting the appropriate irradiation dose in mutation breeding programs in cherry tomato.
Keywords : Etkili mutasyon dozu (EMD50), in vitro, ex vitro, Kiraz domatesi, solanum lycopersicum L. var. cerasiforme

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