Bacteriological and Molecular Characterization of Staphylococcus aureus Isolates from Hair and Saanen Goats

Authors : Deha Ali Deniz, Şükrü Kırkan

Pages : 58-64

Doi:10.53913/aduveterinary.1809128

View : 71 | Download : 92

Publication Date : 2025-12-25

Article Type : Research Paper

Abstract :In this study, a total of 400 samples were collected from the milk, skin, vaginal mucosa, and nasal mucosa of 50 lactating Hair goats and 50 lactating Saanen goats raised in the Aydın province during the summer of 2010. The samples were transported to the laboratory of the Department of Microbiology, Faculty of Veterinary Medicine, Adnan Menderes University. Staphylococcus aureus was isolated from 48 samples (12%). Polymerase chain reaction (PCR) analysis revealed that 13 (27%) of these isolates harbored the sek staphylococcal enterotoxin gene (278-bp fragment). Among the detected enterotoxin genes, 10 (66.7%) were obtained from milk isolates, 2 (22%) from nasal isolates, and 1 (11.1%) from a skin isolate. Distribution by breed showed that 8 (42.1%) of the genes were detected in milk isolates of Hair goats, 2 (25%) in milk isolates of Saanen goats, 1 (5.2%) in a skin isolate of a Hair goat, and 2 (10.5%) in nasal isolates of Hair goats. The higher prevalence of S. aureus in Hair goats may be associated with their extensive breeding practices and increased environmental exposure to the pathogen. These findings emphasize the importance of management, nutrition, and hygiene practices in Hair goat flocks. Furthermore, molecular methods provided rapid and highly sensitive detection of staphylococcal enterotoxin genes.
Keywords : Enterotoksin, Keçi, PCR, Staphylococcus aureus, Süt

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