The Characterization and Activity of Protease Enzyme on Different Days of Tadpoles [Pelophylax ridibundus (Pallas, 1771)]

Authors : Hülya ŞEREFLİŞAN, Ahmet ALKAYA, Kaya GÖKÇEK

Pages : 112-115

Doi:10.26650/ASE2019567360

View : 37 | Download : 8

Publication Date : 2019-09-16

Article Type : Research Paper

Abstract :In this study, protease enzyme activity of Pelophylax ridibundus insert ignore into journalissuearticles values(Pallas, 1771); tadpoles was determined during the larval period starting from 25 Gosner stage insert ignore into journalissuearticles values(1960);. When the P. ridibundus tadpoles reached the 25 Gosner stage insert ignore into journalissuearticles values(1960); on the 22nd day, the sampling was continued until the metamorphosis was completed by sampling at 5-day intervals. Initial average weight and length of the tadpoles were 0.076 g and 6.42 mm, respectively. The tadpoles were fed with pellet feed the size of 2 mm and 39% crude protein content twice a day in the morning and afternoon. The differences in the protease activity between the 22nd day and the 62nd day of the metamorphosis were found statistically significant insert ignore into journalissuearticles values(P<0.05);. It has been observed that the protease enzyme reached maximum level at the 32nd day. In the next stages, protease activity showed a tendency to increase and decrease up to metamorphosis. These results can be used as the basis for the most appropriate nutritional formulation for P. ridibundus in the larval stage.
Keywords : Pelophylax ridibundus, frog, acid protease, larval period, metamorphosis

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