Molecular Phylogenetic Relationships of Some Fabaceae Species That Used As Forage Crops in Turkiye

Authors : Mevlüde Alev Ateş

Pages : 119-123

View : 14 | Download : 11

Publication Date : 2020-12-31

Article Type : Conference Paper

Abstract :Fabaceae which are called pea family, is the 3ʳᵈ largest family of Angiosperms. The family consist of over 20.000 species of 700 genera with trees, shrubs, herbs all over the World. Although, many species of the family have economical values, some species especially herbs are used as forage crops like soybeans, peas, alfa alfa, cowpea, clover, etc. in Turkey. Nowadays, molecular regions of DNA were used to clarify genetic relationships, so they were used in taxonomical studies and in breeding studies while hybridizing species to obtain economically and nutritionally valuable plants. Especially ITS (Internal Transcribe Spacer) region was mostly preferred for molecular phylogenetic studies due to its highly repeated in number in plant genomes and large copy numbers that support PCR amplification. In the study, related sequences were obtained from NCBI database and statistics were analyzed in MEGA X software. Phylogenetic tree was constructed via BEAST program package. According to the phylogenetic tree, species were divided into 2 main clusters. One of the clusters composed of Atriplex and Amaranthus genus which were the members of outgroup family Amaranthaceae. Although Astragalus and Onobrychis genus were located at same sub branch, Pisum, Lathyrus and Vicia genus were positioned close to each other. Therefore, genetic distances among genus and species were clearly identified by checking the phylogenetic tree. Conclusively, both molecular and classical breeding methods could be efficiently applied together for these forage crops in future breeding studies.
Keywords : Fabaceae, ıslah, filogeni, ITS, DNA

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