Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study

Authors : Ugur MERCAN, Z Burcin GONEN, Hasan SALKİN, Gul Merve YALCİN ULKER, Deniz Gokce MERAL

Pages : 67-73

Doi:10.26650/eor.20197456

View : 48 | Download : 9

Publication Date : 2019-05-31

Article Type : Research Paper

Abstract :Purpose The aim of this study is to compare effects of postoperative care agents; chlorhexidine, octenidine dihydrochloride and hyaluronic acid on human gingival fibroblasts’ viability, proliferation, apoptosis and migration. Material and Methods After cell culturing; chlorhexidine, octenidine dihydrochloride and hyaluronic acid solutions were applied on cells and nothing was applied for control group. The cells were monitored to investigate cytotoxicity; the percentage of apoptotic, living and dead cells at the time of 24, 48, and 72 hours insert ignore into journalissuearticles values(h);. A scratch wound assay was performed to detect cell migration and cells were monitored at baseline, at 24 and 48h. Results At 24h, chlorhexidine showed statistically lower percentage of total apoptotic cells’ than octenidine dihydrochloride insert ignore into journalissuearticles values(p=0.049);, hyaluronic acid insert ignore into journalissuearticles values(p=0.049); and control insert ignore into journalissuearticles values(p=0.049);. At 48h, hyaluronic acid showed statistically lower percentage than chlorhexidine insert ignore into journalissuearticles values(p=0.049);, and control insert ignore into journalissuearticles values(p=0.049);. All agents were found to have statistically and significantly more cytotoxic than control. However, there was no difference between experimental groups for proliferation rate. Octenidine dihydrochloride showed statistically negative effects on cell migration than chlorhexidine and hyaluronic acid at 24h. Chlorhexidine and hyaluronic acid maintained migration ability of cells than octenidine dihydrochloride at 48h. Conclusion All agents have similar effects on cell behavior such as viability, apoptosis and cell proliferation. However, octenidine dihydrochloride showed statistically negative effects on migration ability than chlorhexidine and hyaluronic acid.
Keywords : Chlorhexidine, hyaluronic acid, octenidine dihydrochloride, cell viability, cell migration

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