Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)

Authors : Luma Abdalmunim BAKER, Hazha Jamal HIDAYET, Nahida S ALCHALABI

Pages : 56-71

View : 47 | Download : 6

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The research include studying biochemical and genetic molecular study for paraoxonase1 enzyme and identify some biochemical parameters for serum blood samples 68 women with breast cancer and follow-up after take them three doses of chemotherapy and comparative with 50 normal female as control group.The result showed for women with breast cancer a significant increase in insert ignore into journalissuearticles values(TC,MDA ,Total protein, U,A , calcium and BMI); when compared with normal women, the results also showed for women who have taken chemical doses and women with breast cancer significant decreased in insert ignore into journalissuearticles values(PON1, GSH); compared with normal women, the results showed for women who have taken  the first dose significant increase in insert ignore into journalissuearticles values(MDA,TP, Calcium, U.A , BMI);, while second dose and the third dose chemotherapy found significant increase in insert ignore into journalissuearticles values(TP, U.A ,Calcium ); and significant decreased for insert ignore into journalissuearticles values(PON1);. The results included sequential analysis for PON1 enzyme ,it has shown results for genetic analyzed selected exon insert ignore into journalissuearticles values(Exon4);, where DNA extraction for normal women and breast cancer women and taken their doses of chemotherapy and measure DNA concentration and purity using Nano-Drop Spectrophotometric Analysis where the result show the DNA concentration ranged between insert ignore into journalissuearticles values(5.2-68.4); ng/ml where concentration mean for women with breast cancer insert ignore into journalissuearticles values(26.97); ng/ml and insert ignore into journalissuearticles values(17.49); ng/ml for normal women while the first dose 24.74 ng/ml , insert ignore into journalissuearticles values(16.16-9.70);ng/ml for second and third dose respectively . It has also been used PCR technique amplification  and test products PCR analysis of sequential technique by using gel electrophoresis where packet appeared clear without deformation and tested PCR product sequencing for exon4 by capillary automated sequencer and  comparing the  result with the normal sequence of the oxon., the results shows for women have taken first dose deletion mutation called insert ignore into journalissuearticles values(Remove); frame shift mutation clear nitrogen base one lead to change all codons after deletion site so that all amino acid change in poly protien chain which led to the loss of amino acid insert ignore into journalissuearticles values(Tyr.); . and mutation type of  silent mutation third mutation type of substitution mutation insert ignore into journalissuearticles values(Replace amino acid insert ignore into journalissuearticles values(Thr.); by amino acid insert ignore into journalissuearticles values(Ser.);.
Keywords : Paraoxonase1, PON1, Chemotherapy, Molecular genetics

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