Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions

Authors : Fatma KALAYCI YÜKSEK, Defne GÜMÜŞ, Gül İpek GÜNDOĞAN, Aysun UYANIK ÖCAL, Nur ELAGÜL, Mine ANĞ KÜÇÜKER

Pages : 85-93

Doi:10.26650/experimed.1124000

View : 94 | Download : 8

Publication Date : 2022-08-22

Article Type : Research Paper

Abstract :Objective: The role of norepinephrine insert ignore into journalissuearticles values(NE); on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus insert ignore into journalissuearticles values(MRSA); ATTC 43300 was examined in human osteoblast insert ignore into journalissuearticles values(HOB); cells. The effects of NE and/or MRSA on the viability and cell death pathways of HOB cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress insert ignore into journalissuearticles values(H2O2); were analyzed in the presence/absence of NE. Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesion-invasion. The alteration of HOB cell viability was determined by methyl thiazolyl diphenyl-tetrazolium bromide insert ignore into journalissuearticles values(MTT); assay. The death pathways of HOB cells were examined microscopically using acridine orange-ethidium bromide dual staining and dichlorofluorescein-diacetate insert ignore into journalissuearticles values(DCF-DA); dye. The bacterial response to H2O2 was investigated by agar dilution. Results: The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE insert ignore into journalissuearticles values(p<0.0001); while high-level NE induced invasion insert ignore into journalissuearticles values(p=0.013);. HOB cell viability was reduced by MRSA and/or NE insert ignore into journalissuearticles values(p<0.001);. MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells insert ignore into journalissuearticles values(p<0.05);. NE did not alter the bacterial response to oxidative stress. Conclusion: Norepinephrine has different effects on the biological properties of both MRSA and HOB cells.
Keywords : Norepinephrine, MRSA, growth, adhesion invasion, oxidative stress response, HOB cell viability, apoptosis necrosis

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