Histopathological and Serum Biomarkers Analyses in MRONJ due to Periodontal Disease in Rats: Comparison of Zoledronic Acid and Denosumab

Authors : Ceren ÖZBEK, Revan Birke KOCAÜNSAL, Merva SOLUK TEKKEŞİN, Faruk ÇELİK, Hayriye Arzu ERGEN, Ümit ZEYBEK, Kıvanç BEKTAŞ KAYHAN, Meral ÜNÜR

Pages : 8-14

Doi:10.26650/experimed.1190912

View : 34 | Download : 9

Publication Date : 2023-05-11

Article Type : Research Paper

Abstract :Objective: This study aimed to investigate the bisphosphonate and denosumab effects in medication-related osteonecrosis of the jaws insert ignore into journalissuearticles values(MRONJ); caused by periodontal disease with analyses of serum biomarkers and histopathology. Materials and Methods: Forty Copenhagen rats were used in the study. A ligature wire was wrapped around the first molars to induce periodontal disease. The rats were divided into a zoledronic acid group insert ignore into journalissuearticles values(ZG); insert ignore into journalissuearticles values(n=12);, a denosumab group insert ignore into journalissuearticles values(DG); insert ignore into journalissuearticles values(n=12);, a saline group insert ignore into journalissuearticles values(SG); insert ignore into journalissuearticles values(n=10);, and a control group insert ignore into journalissuearticles values(CG); insert ignore into journalissuearticles values(n=6);. Prostate cancer was induced by injections for ZG, DG, and SG following the ligature application, and injections were repeated on the 14th and 21st days. While periodontal disease was evaluated clinically with gingival edema, swelling and redness, serum osteocalcin, osteopontin, parathormone and receptor activator of nuclear factor-kappa B ligand insert ignore into journalissuearticles values(RANKL); levels were evaluated using the LUMINEX technique. The Mann-Whitney U test was used for the comparison of parameters between groups insert ignore into journalissuearticles values(p<0.05);. Results: The osteocalcin levels were increased in CG, RANKL levels were decreased in DG, osteopontin levels were increased in ZG, and parathormone levels were increased in both ZG and CG. Conclusion: Since the long-term use of bisphosphonates can cause osteonecrosis in the jaw bones, it should not be overlooked that this can also be caused by chronic inflammatory conditions such as periodontal disease.
Keywords : Bisphosphonates, denosumab, osteonecrosis, periodontal diseases, rats

ORIGINAL ARTICLE URL