ELECTROSPUN SILK FIBROIN/GRAPHENE NANOFIBERS FUNCTIONALIZED WITH PHOTOSENSITIVE PORPHYRINS: AN ADVANCED BACTERICIDAL PLATFORM FOR TISSUE REGENERATION

Authors : Elçin Tören, Sevda Altaş

Pages : 221-236

Doi:10.7216/teksmuh.1633364

View : 51 | Download : 46

Publication Date : 2025-09-30

Article Type : Research Paper

Abstract :Graphene oxide (GO)functionalised with meso-tetrakis(N-methyl pyridinium-four-yl) porphyrin (TMP) electrospun nanofibers silk fibroin (SF) resulted in a wound recovery and tissue regeneration dual functional platform. The combination of TMP into SF/GOmatrix improves mechanical properties and possesses antibacterial activity and biocompatibility. As revealed through mechanical testing, as TMP concentration was increased, the Young\\\'s Modulus improved, as exhibited by SF/GO/TMP fibres with a peak stiffness of 38 MPa at 1.00 mg/mL TMP and elongation decreased in agreement, demonstrating the potential for an inverse relationship between tension and flexibility. SEM and fibre diameter analyses demonstrated that GO increased the fibre thickness and structural uniformity up to and including an optimal concentration of 200 μg/ml. Antibacterials have demonstrated an inhibitory effect against Staphylococcus aureus (21.4 mm) and Escherichia coli (15.8 mm) by inducing the production of reactive oxygen species (ROS) in the presence of light. MTT assays confirmed the cytocompatibility of nanofibers supporting fibroblast proliferation for 72 h, and haemolysis tests showed a negligible haemolytic potential (0.7%). These results highlight the continued potential of SF/GO/TMP composites as functional wound dressings and scaffolds with improved mechanical stability, antibacterial safety, and biocompatibility, which are required for more advanced biomedical fields.
Keywords : İpek Fibroin, Grafen Oksit, Porfirin, Yara İyileştirme, Bakterisidal Platform, Işığa Duyarlı Porfirinler

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