Experimental Design Optimization and Decolorization of an Azo Dye by Cross-Linked Peroxidase Aggregates

Authors : Fatma AYHAN, Oguz AKPOLAT

Pages : 34-42

Doi:10.32710/tekstilvekonfeksiyon.498774

View : 46 | Download : 17

Publication Date : 2021-03-31

Article Type : Research Paper

Abstract :The immobilization of horseradish peroxidase in the form of cross-linked enzyme aggregates insert ignore into journalissuearticles values(HRP–CLEAs); was optimized by three parameters full factorial experimental design at two levels and the degradation of an azo dye was tested. The optimal immobilization conditions were estimated as 0.06 mg enzyme/mL insert ignore into journalissuearticles values(0.96 U);, 3 % insert ignore into journalissuearticles values(v/v, Glutaraldehyde);); cross-linker ratio, and 6 mg /mL Bovine Serume Albumine amount, respectively. The effects of variables were analysed by responce surface plots and the enzyme concentration effect was insignificant for the tested levels. The maximum CLEAs activity was 0.2188 U and the kinetic parameters insert ignore into journalissuearticles values(K m and V max ); were 0.0314 mM and 0.1044 mM/min while the calculated values for soluble enzyme were 0.06 mM and 0.468 mM/min, respectively. Hydrogen peroxide has inhibitory effect on enzyme aggregate activities at higher amounts than 0.012 mM. Under optimal conditions, a textile azo dye, Reactive Blue 160 was completely oxidized within 90 h and higher temperatures also showed promising results in dye degradation.  
Keywords : Cross Linked Enzyme Aggregates CLEAs, Horseradish Peroxidase, Full Factorial Design, Decolorization

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