Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats

Authors : Muhammet Yasin TEKELİ

Pages : 76-85

Doi:10.32707/ercivet.1332103

View : 98 | Download : 160

Publication Date : 2023-08-01

Article Type : Research Paper

Abstract :This study was aimed to investigate the effects of diosmin insert ignore into journalissuearticles values(DIO); in rats exposed to emamectin benzoate insert ignore into journalissuearticles values(EB);. For this purpose, a total of 60 Wistar Albino male rats, aged 6 to 8 weeks and weighing 180 to 250 g, were used, 10 in each group. The groups were determined, respectively, control, EB insert ignore into journalissuearticles values(10 mg kg-1);, DIO insert ignore into journalissuearticles values(50 mg kg-1);, DIO insert ignore into journalissuearticles values(100 mg kg-1);, EB insert ignore into journalissuearticles values(10 mg kg-1); + DIO insert ignore into journalissuearticles values(50 mg kg-1);, EB insert ignore into journalissuearticles values(10 mg kg-1); + DIO insert ignore into journalissuearticles values(100 mg kg-1);, and the indicated doses were applied by gavage for 21 days. At the end of the trial period, a heart puncture was performed under anesthesia and blood samples were taken into tubes with heparinized and anticoagulant-free properties. The liver, kidney, brain, testis, heart and lung tissues were removed after cervical dislocation. While some biochemical markers, such as aspartate aminotrans- ferase insert ignore into journalissuearticles values(AST);, alanine aminotransferase insert ignore into journalissuearticles values(ALT);, lactate dehydrogenase insert ignore into journalissuearticles values(LDH);, alkaline phosphatase insert ignore into journalissuearticles values(ALP);, triglyceride, cholesterol, total protein, albumin, uric acid, blood urea nitrogen insert ignore into journalissuearticles values(BUN); and, creatinine were assessed in the serum, oxidative stress indicators, such as MDA, NO, SOD, CAT, GSH-Px, GR, GST, and GSH, were determined in tissue samples. At the end of the investigation, no changes between the parameters of the DIO groups and the control group were discovered. Comparing the EB group to the control group, it was found that the levels of MDA and NO in tissues/ erythrocytes had significantly increased. The activity of the GSH, GR, GST, SOD, and CAT enzymes, as well as GSH levels, was found to have decreased. Furthermore, serum total protein and albumin levels were reduced, although AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities were elevated. Depend- ing on the dose, it was found that the values of the groups coadministered with EMB and DIO were close to those of the control group. In conclusion, it is proposed that DIO could provide protection against EMB-induced toxicity in rats.
Keywords : Emamektin benzoat, diosmin, oksidatif hasar, rat

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