INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

S Catalgol; B Catalgol B ALPERTUNGA

INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Authors : S Catalgol, B Catalgol B ALPERTUNGA

Pages : 103-120

View : 100 | Download : 18

Publication Date : 2014-03-24

Article Type : Research Paper

Abstract :SUMMARY This study aimed to evaluate the role of oxidative stress in toxicity induced by BNL (benomyl) and its metabolite CBZ (carbendazim), which are systemic broad-spectrum benzimidazole fungicides. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyl transpeptidase (GGT) and glutathione-S-transferase (GST) as well as the levels of malonyldialdehyde (MDA), being a lipid peroxidation marker and reduced glutathione (GSH) were measured in the liver, kidney, testis and brain tissues of the rats, after acute exposure of the two fungicides and their mixture. In all tissues, it was observed that MDA levels were increased and changes in antioxidant defense system were occurred. The induction of oxidative stress manifested as changes in the activity and/or levels of antioxidative parameters has been suggested as the mechanism by which BNL and CBZ induces their toxic effects. ÖZET Bu çalışmada, geniş spektrumlu sistemik benzimidazol grubu fungusidlerden olan BNL (benomyl) ve metaboliti CBZ (carbendazim)’ nin toksik etki mekanizmalarında oksidatif stresin rolü araştırıldı. BNL, CBZ ve karışımlarına akut maruziyet sonrası sıçanların karaciğer, böbrek, testis ve beyin dokularında oksidatif stres göstergesi olarak lipid peroksidasyon son ürünü malondialdehid (MDA) ve indirgenmiş glutatyon (GSH) seviyeleri, süperoksid dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx), glutatyon redüktaz (GSH-Rd), gama glutamil transpeptidaz (GGT) ve glutatyon-S-transferaz (GST) antioksidan enzim aktivitelerinin tayini yapıldı. Tüm dokularda MDA düzeylerinin arttığı ve antioksidan savunma sistemlerinde değişiklik olduğu gözlendi. BNL ve CBZ’nin toksisitesinde, antioksidan parametrelerin aktivite ve/veya düzeylerinde oksidatif stresin indüksiyonu sonucu görülen değişikliklerin rol oynadığı sonucuna varıldı. Key words: Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity
Keywords : Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

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