Investigation of the Effects of Polydatin on Gentamicin-Induced Renal Toxicity in Rats

Authors : Özge Kandemir, Hasan Şimşek, Nurhan Akaras, Fatih Mehmet Kandemir

Pages : 280-289

Doi:10.30607/kvj.1670028

View : 65 | Download : 72

Publication Date : 2025-10-03

Article Type : Research Paper

Abstract :Gentamicin (GNT), an aminoglycoside antibiotic, induces nephrotoxicity through mechanisms like tubular apoptosis and inflammation. Polydatin (Poly), a natural polyphenolic compound with antioxidant and anti-inflammatory properties, has shown potential in alleviating renal damage. This study aimed to investigate the protective effects of Poly in rats with GNT-induced kidney injury using biochemical, molecular, and histopathological methods. 35 Wistar albino rats were divided into 5 groups (7 rats/group), including control, Poly (100 mg/kg), GNT (100 mg/kg), and two combined treatment groups (GNT+Poly at 50 mg/kg and 100 mg/kg). After 7 days of treatment, kidney tissues and blood were collected for analysis of renal function markers, oxidant-antioxidant parameters, gene expression (NF-κB, TNF-α, Caspase-3, Bax, Bcl-2, KIM1, AQP2), and histopathological evaluation. GNT increased serum urea and creatinine levels (p<0.001), increased MDA levels (p<0.001) and decreased antioxidants (p<0.001); also increased the expression of NF-κB and TNF-α (p<0.001), increased Caspase-3 and Bax (p<0.001) and decreased Bcl-2 levels (p<0.001). When administered together with GNT, Poly decreased MDA levels (p<0.001) and increased GSH levels (p<0.001), decreased inflammation markers (NF-κB and TNF-α) (p<0.01), decreased Caspase-3 and Bax (p<0.01) and increased Bcl-2 levels (p<0.01), and also improved histological damage and decreased histological score (p<0.05). In GNT-induced renal toxicity, Poly 100 treatment provided renal protection by reversing oxidative stress, inflammation, and apoptosis.
Keywords : Apoptozis, gentamisin, nefrotoksisite, oksidatif stres, polidatin

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