Hyperhomocysteinemia Transcriptionally Regulates Expression of a Set of Ion Channels in Brain and Heart Tissues in Mice

Authors : Ebru Önalan, İlay Buran Kavuran, Ahmet Tektemur, Esin Öz, Seda Özaydın, Arzu Etem Akağaç, Ramazan Bal

Pages : 12-19

Doi:10.47572/muskutd.1347282

View : 95 | Download : 66

Publication Date : 2024-04-30

Article Type : Research Paper

Abstract :The alterations of ion channel gene expressions in brain and heart tissues in HHcy have not been previously reported. We investigated the mRNA expression levels in brain and heart tissues of the HHcy mice compared to the control mice to characterize distinct expression of 36 ion channels. C57BL/6 J. mice were divided into two groups of 15 animals each: (1) control group and (2) HHcy group. The HHcy was induced by methionine administiration. The mRNA levels of ion channels were analyzed using qRT-PCR. TUNEL staining and MDA assay were used for verification of the negative side effects of HHcy in heart and brain tissues. RT-PCR revealed the upregulation of Hcn4, Trpc3, Trpm2 and the downregulation of Abbc8, Cacna1b, Cacna1c, Cacna1e, Cacna1h, Hcn1, Kcnc3, Kcnh7, Kcnj8, Trpc4, Trpc5, Trpc6, Trpm3, Trpm4, Trpv4, Trpv6 in brain tissues of the HHcy group compared to the control. The upregulation of ion channel expressions in heart tissues were not detected, but we found only the downregulation of Accn1, Accn2, Accn3, Hcn1, Kcnc4 and Trpv6 ion channels. Apoptozis and MDA level were significantly increased in brain and heart tissues of the HHcy group compared to the control. Brain tissues compared to heart tissues exhibit a very considerable and diverse ion channel gene expression pattern in mice with HHcy than control. Clarifying the roles of ion channels in HHcy could shed light on the development of novel therapeutic strategies and ultimately improve HHcy side effects.
Keywords : Asit Duyarlı İyon Kanalları, Geçici Reseptör Potansiyel Kanalları, Hiperhomosisteinemi, Kalsiyum Kanalları, Potasyum Kanalları

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