Effect of Blood Sample Tubes on Diagnosis of Gestational Diabetes in Pregnant Women Undergoing OGTT

Authors : Duygu Teksöz, Nilgün Işıksaçan, Tamay Seda Taşçı, Pınar Kasapoğlu, Ramazan Murat Koşer

Pages : 165-172

Doi:10.47572/muskutd.1479380

View : 34 | Download : 11

Publication Date : 2024-12-18

Article Type : Research Paper

Abstract :Performing a diagnostic oral glucose tolerance test (OGTT) during pregnancy is important for early diagnosis of gestational diabetes mellitus. Hyperglycemia can be detected quickly, and mother and the fetus can be protected. This study aimed to research the relationship between blood sample tubes and the stability of glucose in pregnant women undergoing OGTT. Blood samples were taken at the 120th minute after glucose loading from 20 pregnant volunteers who underwent OGTT (75g). VACUETTE CAT Serum Separator (Clot Activator Tube) was used for serum; VACUETTE FC Mix tube (Na2 EDTA, sodium fluoride, sodium citrate) and VACUETTE FE (Sodium Fluoride/K3 EDTA) were used for plasma. All three of these tubes were centrifuged at three different times i.e. 0. hour, 2. hour, and 4. hour. All samples were centrifuged at 0., 2., and 4. hours, and glucose values were measured. No statistically meaningful difference was found between their glucose values in only VACUETTE FC Mix tubes (p>0.05). However, a statistically meaningful difference was found at these different time intervals in serum and fluoride tubes (p<0.01). It was shown that when the blood sampled into VACUETTE FC Mix was kept without centrifugation even at the 4. hour, their glucose values did not decrease and therefore the routine use of them would be beneficial. However, it was found that the glucose values were not stable in VACUETTE CAT Serum Separator and VACUETTE FE.
Keywords : Gebelik, Gestasyonel Diyabet, Glikolizis, Glukoz, Oral Glukoz Tolerans Testi

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