Gibberellic Acid is Active Only in Orchid Cross-Pollination

Authors : Mehmet Aybeke

Pages : 533-542

Doi:10.47495/okufbed.1452135

View : 50 | Download : 47

Publication Date : 2025-03-12

Article Type : Research Paper

Abstract :The aim of this study is to determine the endogenous hormone activities of Gibberellic acid (GA) in compatible (Intraspecific= ISP) and incompatible pollination (Intergeneric= IGP). It was designed to be Himantoglossum robertianum in ISP experiments and Himantoglossum robertianum and Orchis italica in IGP experiments. For this reason, some polynariums taken from O. italica have been applied with needles to the flower stigmas of H. robertianum. Ovaries with stigma have been taken from both types of pollination for 10 days, and quantitative hormone analyses have been performed by LC-MS/MS. As a result, GA was not found in the ISP experiments. Likewise, it did not appear at all in the tests between the 1st and 6th days of IGP; it was found only on the 7th, 8th, 9th, and 10th days of IGP and at increasingly higher values. For the first time, endogenous ovary-stigma GA amounts in the post-pollination process of orchid IGP were determined in this study, and its importance was discussed. According to statistical analysis, there is a significant difference between almost all values. It has been understood that additional histological and embryological studies are needed to understand the reason for the very high activity, especially on days 9 and 10.
Keywords : Himantoglossum, hormon, orkide, tozlaşma sonrası olaylar, çiçek