A Potential Common Pyrroline-5-Carboxylate Synthetase (P5CS) Gene As A Stress Marker For qRT-PCR In Legume Plants

Authors : Hande Morgil

Pages : 739-753

Doi:10.47495/okufbed.1569244

View : 38 | Download : 66

Publication Date : 2025-03-12

Article Type : Research Paper

Abstract :Leguminosae (or Fabaceae), commonly known as legumes and including plants of great agricultural and ecological importance, are important agricultural plant families with a very wide distribution worldwide. Stress is one of the main environmental factors affecting the growth and productivity of cultivated plants. Plants are designed to minimize the impact of stress and to stress resistance at the biochemical level by producing osmolytes such as proline to protect its components. Pyrroline-5-carboxylate synthetase (P5CS) is a key enzyme in the proline biosynthesis pathway in plants and is involved in the initial step of proline production. In this study, a common primer was identified to enable qRT-PCR-based studies as an alternative to biochemical methods for determining the increase in proline content. With this aim, important agricultural plants, Phaseolus vulgaris, Lens culinaris, Cicer arietinum, Glycine max, Pisum sativum, Medicago sativa, were exposed to three different stresses (drought, heat, salt). Proline amounts of the samples were determined biochemically and then gene expression levels were calculated using the common primer. In our results, proline levels were significantly increased in all plant samples. Relative gene expression analysis results also correlated with the biochemical results. In conclusion, this study revealed the existence of a common marker that can be used to determine proline levels at the gene expression level, which is an important marker indicating that some agricultural legume plants are under stress. Our findings show that investigating the expression of genes involved in proline biosynthesis can help to understand the stress responses of plants at the molecular level. In addition, analysis, especially by molecular methods, provides faster and more precise results. This allows to observe the dynamic changes of genes active in proline biosynthesis in a shorter time and changes at the gene level allow us to observe the plant\\\'s readiness or early responses to stress.
Keywords : Leguminosae, Abiyotik stres, Prolin biyosentezi, Pirolin-5-karboksilat sentetaz (P5CS), Gen ekspresyonu