Growth and aflatoxin production of Aspergillus flavus in fig-based medium: Effect of minerals and chelating agents

Authors : Sule GUNAYDIN, Hakan KARACA

Pages : 1223-1233

View : 18 | Download : 8

Publication Date : 2020-12-07

Article Type : Research Paper

Abstract :In this study, minerals required for the growth and aflatoxin production of A. flavus were determined. Then, the effectiveness of some chelating agents such as citric acid, phytic acid and ethylenediaminetetraacetic acid insert ignore into journalissuearticles values(EDTA); in preventing mold growth and toxin production was investigated in a fig-based medium and in two synthetic media, called Czapek-dox agar insert ignore into journalissuearticles values(CZA); and potato dextrose agar insert ignore into journalissuearticles values(PDA);. A. flavus did not grow in CZA without sodium nitrate during 6-day incubation at 30 °C. The absence of the other components insert ignore into journalissuearticles values(magnesium sulfate, iron sulfate, potassium chloride and potassium phosphate); did not significantly insert ignore into journalissuearticles values(p>0.05); affect the growth of the mold. However, the mold produced significantly insert ignore into journalissuearticles values(p<0.05); higher amounts of aflatoxins in the media without magnesium sulfate and iron sulfate. A. flavus grew faster on fig-based medium and produced higher amounts of aflatoxins in PDA. In all media tested in this study, EDTA was found to be more effective than the other agents in controlling the growth of A. flavus. It was also effective in inhibiting the production of aflatoxins. It was determined that 1.75 mM EDTA reduced aflatoxin production up to 97% in PDA. Citric and phytic acids could inhibit the growth of the mold only in PDA. All chelating agent reduced aflatoxin production over 90% in fig-based medium.
Keywords : Template mycotoxin, mold, Ethylenediaminetetraacetic acid, Citric acid, Phytic acid, Aflatoxin, Aspergillus flavus