Protective Effects of Octreotide on Acetaminophen-Induced Liver Damage in Rats: Biochemical and Histopathological Evaluation

Authors : Murat Akbulut, Semih Tan, Hülya Çetin, Saim Özdamar

Pages : 441-450

Doi:10.34108/eujhs.1671907

View : 48 | Download : 166

Publication Date : 2025-12-30

Article Type : Research Paper

Abstract :This study aimed to evaluate the potential protective effects of octreotide, a somatostatin analogue, against acute liver injury induced by acetaminophen in rats. 28 male Wistar albino rats, aged 8–10 weeks, were randomly assigned into four experimental groups. Group 1 served as the control, and Group 2 received 1 g/kg of acetaminophen orally, Group 3 was administered 300 µg/kg of octreotide intraperitoneally, Group 4 was treated with both agents, where octreotide was given 30 minutes after acetaminophen administration. The experiment was terminated by taking liver tissue and blood samples under general anesthesia 24 hours after drug administration. Hematoxylin and Eosin staining was performed on the liver sections, and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, as well as total antioxidant capacity, total oxidant capacity and malondialdehyde levelswere analyzed. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, and total oxidant capacity were significantly higher in acetaminophen-treated rats compared to the other groups. Similarly, malondialdehyde levels were also higher, but these values were not statistically significant. Total antioxidant capacity were lower in the acetaminophen group compared to the other groups. In contrast, the treatment group showed a significant reduction in aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities and total oxidant capacitycompared to the acetaminophen group. Histological analysis revealed severe hepatic damage in the acetaminophen group, while less tissue damage was noted in the treatment group. Although malondialdehyde was lower in the treatment group on average, the difference was not statistically significant. These findings suggest that octreotide may offer partial protection against acetaminophen-induced liver injury. However, further investigation is required to elucidate its precise mechanism of action.
Keywords : Asetaminofen, karaciğer hasarı, oktreotid, oksidatif stres

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