Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats

Authors : Fatma ÖZDEMİR, Seza ARSLAN

Pages : 1674-1682

Doi:10.16984/saufenbilder.395016

View : 21 | Download : 9

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The genus Bacillus is frequently found in soil, water and food. Bacillus cereus and Bacillus anthracis are the main pathogens causing foodborne diseases and serious infections in humans. A total of 52 Bacillus spp. from meat samples was tested for determination of biofilm production, antimicrobial resistance pattern and beta-lactamase activity. The 24 insert ignore into journalissuearticles values(46.1%); Bacillus isolates were found to be for biofilm production. Of the 24 insert ignore into journalissuearticles values(46.1%); biofilm producer Bacillus isolates, 13 insert ignore into journalissuearticles values(25%);, 6 insert ignore into journalissuearticles values(11.5%); and 5 insert ignore into journalissuearticles values(9.6%); were considered as strong, moderate and weak biofilm producer, respectively. The most common species for the production of biofilm was Bacillus thuringiensis insert ignore into journalissuearticles values(80%);. Antimicrobial disk susceptibility tests of Bacillus spp. revealed high resistance to ampicillin insert ignore into journalissuearticles values(84.6%); followed by penicillin insert ignore into journalissuearticles values(75%);, cefepime insert ignore into journalissuearticles values(34.6%);, and cefoxitin insert ignore into journalissuearticles values(26.9%);. A multidrug resistance to at least 3 or more antimicrobials was observed in the 25 isolates insert ignore into journalissuearticles values(48.1%);. All Bacillus spp. were sensitive to vancomycin, gentamicin, amikacin, ciprofloxacin, and imipenem. The susceptibility rate to streptomycin, chloramphenicol, and trimethoprim-sulphamethoxazole was 94.2%. Among the isolates, the 6 insert ignore into journalissuearticles values(11.5%); isolates were found to be sensitive to all antimicrobial agents tested. Besides, only one isolate from meat was found to be positive for beta-lactamase test. The existence of biofilm production as a virulence factor and of multidrug resistance in bacteria isolated from food should not be underestimated in terms of food safety, public health and economic concerns.
Keywords : Bacillus spp, biofilm production, antimicrobial resistance, beta lactamase