Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Authors : Ertan Ermiş, Ecem Poyraz, Enes Dertli, Mustafa Tahsin Yılmaz

Pages : 151-160

Doi:10.16984/saufenbilder.545929

View : 21 | Download : 9

Publication Date : 2020-02-01

Article Type : Research Paper

Abstract :Response surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables, temperature insert ignore into journalissuearticles values(30, 36 and 42 °C);, incubation time insert ignore into journalissuearticles values(18, 33 and 48 h); and initial pH of growth medium insert ignore into journalissuearticles values(4.5, 5.5 and 6.5); were studied. Under optimum growth conditions, the amount of exopolysaccharide derived from Lactobacillus brevis E25 ranged from 10 to 35 gL -1 . The size of EPS molecules ranged from 10 5 to 10 7 Da. Infra red spectrum analysis showed characteristics absorption peaks indicating the presence of -OH, C=O and C-H groups. Furthermore, only glucose was detected as monosaccharide in exopolysaccharide structure, revealing that the structure of exopolysaccharide is a homopolymeric glucan type . Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.
Keywords : Lactic acid bacteria, exopolysaccharide, response surface methodology, molecular characterization