Effect of Mitosis on the Resistance to Oxidative and Osmotic Stresses in Yeast

Authors : Pinar Buket ATALAY, Nur KALUC, Elif Ergin CAVUSOGLU

Pages : 265-271

Doi:10.16984/saufenbilder.559820

View : 52 | Download : 11

Publication Date : 2020-02-01

Article Type : Research Paper

Abstract :Cancer cells are defined by abnormal and unrestricted mitotic divisions, therefore targeting mitosis is a useful strategy for cancer treatment. Two groups of drugs that are most successfully used in the treatment of several types of cancer, taxanes and vinca alkaloids, exhibit their anti-tumor effects by causing a mitotic arrest. However, not much is known about whether being arrested in mitosis affects the sensitivity of cells to tumor-related stresses, such as oxidative and osmotic stresses. In this study, we investigated whether mitosis affects the sensitivity of S accharomyces cerevisiae cells to H 2 O 2 -induced oxidative stress and sorbitol-induced osmotic stress. Mitotic and G1-arrests were induced by nocodazole and alpha factor, respectively. The effects of nocodazole or alpha factor treatments on the sensitivity of wild type insert ignore into journalissuearticles values(WT); and MAD3 deletion insert ignore into journalissuearticles values( mad3Δ ); strains to oxidative stress and osmotic stress were evaluated by the spotting and coloni forming unit insert ignore into journalissuearticles values(cfu); assays as well as detection of reactive oxygen species insert ignore into journalissuearticles values(ROS); production. Data were analyzed using Student’s t-test and expressed as standard deviation insert ignore into journalissuearticles values(std);, p <0.05 was considered significant. Our data indicate that mitosis significantly increases resistance to oxidative stress , however it does not have any significant effect on the osmotic stress resistance in yeast.
Keywords : Oxidative stress, Osmotic stress, Mitosis, Saccharomyces cerevisiae

ORIGINAL ARTICLE URL