Mediator effect of luteolin on electrooxidation of NADH

Authors : Melike BİLGİ KAMAÇ, Gulshat GYLYJOVA

Pages : 453-465

Doi:10.16984/saufenbilder.745610

View : 20 | Download : 11

Publication Date : 2021-04-15

Article Type : Research Paper

Abstract :The effects of luteolin insert ignore into journalissuearticles values(LU); as a new mediator on the electrooxidation of the reduced nicotinamide adenine dinucleotide insert ignore into journalissuearticles values(NADH); were investigated and developed a new disposable NADH sensor. Firstly, screen printed carbon electrodes insert ignore into journalissuearticles values(SPCE); modified with gold nanoparticles insert ignore into journalissuearticles values(AuNP);, and then, LU was deposited on SPCE modified with AuNP insert ignore into journalissuearticles values(SPCE/AuNP); using cyclic voltammetry. Electrochemical behavior of LU on SPCE/AuNP was investigated and, the redox proses of LU on SPCE/AuNP was found to be adsorption controlled. The number of cycles was optimized for the electrochemical deposition of LU and determined to be 15 cycles. LU modified SPCE/AuNP insert ignore into journalissuearticles values(SPCE/AuNP/LU); was found to show the electrocatalytic effect on electrooxidation of NADH and acts as a mediator. Working potential and pH were optimized for electrochemical detection of NADH with the proposed SPCE/AuNP/LU sensor and found to be +0.225 V and 7.25, respectively. The proposed NADH sensor showed a well linear response from 15.90 to 925 μM with a detection limit of 4.57 μM and a sensitivity of 11.19 μAmM−1. The repeatability of the NADH sensor was tested +0.225 V with 50 µM NADH solution. The relative standard deviation insert ignore into journalissuearticles values(RSD); was calculated as 3.68% insert ignore into journalissuearticles values(for n=10);. The operational stability studies have shown that the initial amperometric response of sensor to NADH decreased by 62.1% at the 30th day. The analysis of NADH in artificial human serum samples was successfully performed with SPCE/AuNP/LU sensor.
Keywords : NADH, luteolin, gold nanoparticles, redox mediator, human blood serum