Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves

Authors : Nergis KAYA, Tayfun KAYA, Soner YİĞİT

Pages : 22-38

Doi:10.16984/saufenbilder.1132730

View : 21 | Download : 6

Publication Date : 2023-02-28

Article Type : Research Paper

Abstract :The antioxidant defense system of the medicinal plant Raphanus sativus L. tuber root in terms of certain antioxidant enzymes insert ignore into journalissuearticles values(ascorbat peroxidase, glutathion reductase, catalase, superoxide dismutase); and lipid peroxidation against gray mold disease caused by Botrytis cinerea fungus on Vicia faba L. insert ignore into journalissuearticles values(bean); leaves was analyzed spectrophotometrically. V. faba plants were grown from seed under controlled conditions. A spore suspension of 105 spores/ml of B. cinerea was prepared. The leaves of V. faba were treated with 1-); only distilled water, 2-);only DMSO, 3-); only B. cinerea insert ignore into journalissuearticles values(positive control);, 4-); only extract applications insert ignore into journalissuearticles values(distilled water, ethanol and methanol extract);,5-); B. cinerea treatment after extract applications insert ignore into journalissuearticles values(extract:fungus);. The leaves of V. faba were harvested 24 and 48 hours after the applications. Analyzes of the supernatants obtained from the extracts were performed spectrophotometrically. It has been revealed that the activity of all investigated enzymes generally increased in the extract:fungus application groups compared to the fungus application group. It was determined that the application of fungus alone caused an increase in the activity of enzymes compared to application groups other than the extract:fungus application groups. It was observed that there was an increase in lipid peroxidation insert ignore into journalissuearticles values(MDA); only in the fungus application group compared to all extract:fungus application groups except 10mg/ml distilled water extract:fungus treatment. As a result of the research, it was determined that the extract:fungus applications generally increased the antioxidant enzyme activity levels and decreased the MDA content when compared to the negative control and positive control groups. In extract:fungus applications, enzyme activities were obtained from 10mg/ml distilled water extract:fungus, 10mg/ml EtOH extract:fungus, 10mg/ml MeOH extract:fungus applications. From this point of view, it can be stated that R. sativus distilled water, ethanol and methanol extracts generally give an increased antioxidant defense response in V. faba leaves compared to B. cinerea inoculation alone.
Keywords : Vicia faba L, Raphanus sativus L, Botrytis cinerea, antioxidant enzymes, lipid peroxidation