CLINICOPATHOLOGIC VALUE OF IMMUNOHISTOCHEMICAL PINCH/LIMS-1 ANTIBODY EXPRESSION IN OVARIAN SEROUS CARCINOMA AND BORDERLINE SEROUS TUMOR

Authors : Onur ERTUNÇ, Özlem ERDEM, Zümrüt Arda KAYMAK

Pages : 592-601

Doi:10.17343/sdutfd.1349330

View : 46 | Download : 43

Publication Date : 2023-09-23

Article Type : Research Paper

Abstract :Objective Particularly interesting cys-his rich protein insert ignore into journalissuearticles values(PINCH/ LIMS-1);, a protein implicated in cell adhesion, is assumed to oversee the development and invasion of cancer cells in tumors and tumor-associated stroma. This study aimed to assess PINCH-1 expression in serous borderline tumor insert ignore into journalissuearticles values(SBT); and serous carcinoma insert ignore into journalissuearticles values(SC); in the tumor and peritumoral stroma and scrutinize any associations between its expression and various clinical and pathological parameters. Material and Method In this study, the expression of the PINCH-1 antibody was analyzed in 21 cases of SBT and 89 cases of SC using the indirect immunoperoxidase technique with streptavidin/HRP-biotin. The staining pattern of PINCH-1 in the tumor and peritumoral stroma was evaluated using a semiquantitative scoring method. The staining procedure used in the study allowed for the accurate identification of PINCH-1 expression, and the data obtained through the semiquantitative scoring method provided a reliable of assessing the degree and intensity of PINCH-1 staining. Thus, the correlation between PINCH-1 expression and various pathologic factors such as patient age, tumor size, FIGO stage, intra-abdominal washing cytology, capsule invasion, tumor location in the ovary, tumor grade, and cancer antigen 125 insert ignore into journalissuearticles values(CA125); levels at the time of diagnosis was examined. Results The study found that PINCH-1 was more prevalent in cases of SC than in SBT cases. The tumors in SC cases had stronger staining than those in SBT cases insert ignore into journalissuearticles values(p<0.001);. The study also found a positive correlation between the diffusiveness and intensity of PINCH-1 staining in the tissue surrounding the tumor and whether the tumor was located on one or both sides of the ovaries insert ignore into journalissuearticles values(p = 0.038 for diffusiveness, p = 0.024 for intensity);. However, there was a negative correlation between PINCH-1 staining and tumor size insert ignore into journalissuearticles values(p=0.019 for diffusiveness, p=0.007 for intensity);. Furthermore, the intensity of PINCH-1 tumor staining exhibited statistical significance in Figo stage and tumor grades as these increased insert ignore into journalissuearticles values(p = 0.032 and p = 0.001, respectively);. Conclusion The results of this study indicate that SCs exhibit a higher level of PINCH-1 staining intensity than SBTs. Furthermore, an increase in the FIGO stage and tumor grade is associated with increased intensity of PINCH-1 staining in the tumor tissue. Additionally, the diffusiveness and intensity of PINCH-1 staining in the peritumoral stroma is more remarkable in cases of bilateral tumors but is inversely correlated with tumor size. The observed association between PINCH-1 expression and important clinicopathologic factors suggests that this molecule may be involved in developing serous ovarian cancer. Overall, these findings may contribute to a better understanding of the role of PINCH-1 in ovarian tumorigenesis and may have implications for developing novel therapeutic strategies targeting this pathway.
Keywords : FIGO evresi, PINCH LIMS 1, Seröz borderline tümör, Seröz karsinom, Seröz over karsinomunda PINCH ekspresyonu