mRNA Transcription Analyses of ROS Genes of Olea europaea L. In Vitro Cultures Treated with Different Boron Salts

Authors : Onur Çelik, Ergun Kaya

Pages : 24-32

Doi:10.18615/anadolu.1457244

View : 44 | Download : 45

Publication Date : 2024-06-30

Article Type : Research Paper

Abstract :Various factors such as biotic and abiotic stresses have effects on plant metabolism, development, and growth. Plants have many complex and extraordinary strategies to adapt, defend, avoid and tolerate all these stress conditions. In this study, the relative mRNA levels of antioxidant enzymes of olive, which is very difficult to reproduce under in vitro conditions, were assessed under oxidative stress conditions, after treatment with boron compounds. In this context, three different compounds of the element boron, which are known to affect the ascorbate-glutathione pathway, were added separately at two different concentrations to the nutrient medium of olive under in vitro conditions. As a result of the study, it was observed that the relative mRNA expression levels of antioxidant enzymes such as catalase, ascorbate peroxidase, and superoxide dismutase decreased only in the H3BO3 group among the experimental groups. An increase in the relative mRNA expression levels of antioxidant enzymes was observed in the NaBO2 and ZnBO3 groups compared to the control group. This situation was interpreted as due to an increase in salinity stress which thereby increased the oxidative stress of the applied NaBO2 and ZnBO3 groups. However, in the H3BO3 group, although the concentration was increased twofold, a decrease was observed in the relative mRNA expression levels of the antioxidant enzymes examined. This reveals that application concentration, as well as the compound used, is extremely important.
Keywords : Askorbat peroksidaz, borik asit, katalaz, sodyum metaborat, süperoksit dismutaz