Investigation Biocatalysts of Immobilized Enzyme on New Supports with Ferri and Ferro Nuclei

Ferri and ferro coordination polymers in sphere structure were synthesized. Scanning Electron Microscopy insert ignore into journalissuearticles values(SEM); Energy Dispersive X-Ray Spectroscopy insert ignore into journalissuearticles values(EDX);, Gel Permeation Chromatography insert ignore into journalissuearticles values(GPC);, elemental analysis, and Fourier Transform Infrared Spectroscopy insert ignore into journalissuearticles values(FT-IR); were performed for chemical and structural characterization of the coordination polymers. Glucose oxidase insert ignore into journalissuearticles values(GOD); enzyme immobilized to compare of kinetic parameters deal with glucano-1,5 lacton formation. Analyses results illustrate that structures coordination of ions Fe2+ and Fe3+ are different to the same support. It was seen that 2 mol of Fe2+ ion insert ignore into journalissuearticles values(insert ignore into journalissuearticles values(PS-N-insert ignore into journalissuearticles values([Feinsert ignore into journalissuearticles values(CN);4L]K3);2); was bound per unit structure while 1 mol of Fe3+ ion insert ignore into journalissuearticles values(PS-N-insert ignore into journalissuearticles values([Feinsert ignore into journalissuearticles values(CN);2L]K);); is attaching. Km values of were found as 15.32 and 10.93 for insert ignore into journalissuearticles values(PS-N-Fe2+);-GOD and insert ignore into journalissuearticles values(PS-N-Fe3+);-GOD, respectively. Km value for insert ignore into journalissuearticles values(PS-N-Fe3+);-GOD was found to be 0.5 times higher, possible reason of such a case is the larger reduction potential of Fe3+. As the charge on the coordination structure increased, the enzyme\`s affinity for the substrate increased. After 20 repeated measurements, GOD immobilized on insert ignore into journalissuearticles values(PS-N-Fe3+); polymer retained 45.47% activity, while GOD immobilized on insert ignore into journalissuearticles values(PS-N-Fe2+); polymer retained 57.86% activity.